Evaluation efficiency and phenotypic analysis to ITS-2gene targeting sample from patient infested with scabies. (S. scabiei Var.hominis).in Thi-Qar province/Iraq

The second internal transcribed spacer region of the nuclear ribosomal gene cluster (ITS2). This consists three genes (18S rDNA, 5.8S rDNA and 28S rDNA) which have into RNA. Nevertheless, not translated protein. It is sensitive to detect S. scabiei DNA even after treatment. In addition, this can genetic variations species that are related taxonomically. study aimed determine compare sensitivity specificity ITS-2, scabiei, microscopic examination scabies skin scrapings. employs, ITS2 as a powerful tool for studies intraspecific variation phylogenies closely species. results PCR diagnosis .var .hominis was,(50%) total sample Frome .25,(50%) mite-positive 25(50%) mite-negative ,50%,Sensitivity 50% specificity, Positive predictive value (60%),negative (40%), reached between (30% 60%) globally, ITS-2 suitable phylogenetic astigmatic mites. shows comparatively higher diverse mites lower distinction within These located in tree based on their features>